Portail de l'école GC2ID

You are here: Home » Equipe d'Accueil des Doctorants » VIEILLISSEMENT » Laboratoire de Radiopathologie - CEA - DSV/IRCM/SCSR

Laboratoire de Radiopathologie - CEA - DSV/IRCM/SCSR

Laboratoire de Radiopathologie CEA - DSV/IRCM/SCSR

Responsable de l'unité : P.H. ROMEO

CEA

Discipline principale : Vieillissement

François BOUSSIN

Téléphone : 01 46 54 97 91

boussin@cea.fr

"Réponse des progéniteurs nerveux aux radiations ionisantes et au peptide b-amyloïde", Pascal MILLET. Directeur de thèse : F. BOUSSIN.

"Rôle des cellules endothéliales dans la régulation la neurogenèse". Céline Mathieu, Dir de Thèse : F. Boussin

Neurogenesis persists in the adult brain subventricular zone (SVZ) where neural stem/progenitor cells (NSPC)s lie close to brain endothelial cells (BECs). We investigated whether BECs influence the proliferation of NSPCs. We show that coculture with BECs induces both the exit of NSPCs from the cell cycle (i.e. their quiescence) and activation of their bone morphogenic protein (BMP)/Smad signaling pathway. We demonstrate that BECs produce BMPs and that purified BMP4 itself induces NSPC quiescence. Levels of the phosphatidylinositol phosphatase PTEN were elevated by coculture of NSPCs with BECs, with a concomitant reduction in Akt phosphorylation. Silencing Smad5 with siRNA, or administration of the BMP agonist Noggin in vitro or in vivo, demonstrated that the increase in PTEN in NSPCs requires BMP/Smad signaling and that both pathways regulate NSPC quiescence. BECs may thus ensure long-term neurogenesis by inducing NSPC quiescence so preventing exhaustion of the NSPC pool.